IL-7 Kit ELISA

N° du produit ABIN921098

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IL-7. Il a été cité dans 1 publication.

Aperçu rapide pour IL-7 Kit ELISA (ABIN921098)

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Épitope: AA 26-177

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA)

Détail du produit IL-7 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

Fonction: Sandwich Human IL-7/Interleukin-7 ELISA Kit to quantitate Human IL7 in cell culture supernatants, serum and plasma (EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: D26-H177

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 5.8%, 6.9%, 4.7%
Inter-Assay Precision (CV%): 6.7%, 6.9%, 5.5%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IL-7 Kit ELISA (ABIN921098)

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." dans: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Détail du antigène

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: IL7

Sujet:

Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.

Gene Full Name: interleukin 7

ID gène: 3574

UniProt: P13232

Pathways: Signalistation JAK/STAT