IL1RN Kit ELISA

N° du produit ABIN921100

Détail du produit IL1RN Kit ELISA

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Épitope: AA 26-177

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1RA

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: R26-E177

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: R26-E177

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: The intracellular form of IL1RN is predominantly expressed in epithelial cells.

Plaque: Pre-coated

Protocole: human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-1RA standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 80, Standard deviation: 4.64, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 574, Standard deviation: 25.83, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 1395, Standard deviation: 61.38, CV(%): 4.4,
• Sample 1: n=24, Mean(pg/ml): 76, Standard deviation: 7, CV(%): 9.2
• Sample 2: n=24, Mean(pg/ml): 509, Standard deviation: 37.16, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 1213, Standard deviation: 77.6, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL1RN Kit ELISA (ABIN921100)

Esfahani, Saidijam, Najafi, Goodarzi, Movahedian: "The effect of salusin-β on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)." dans: ARYA atherosclerosis, Vol. 14, Issue 1, pp. 1-10, (2018) (PubMed).

McCarthy: "Management. The name game." dans: Nursing times, Vol. 85, Issue 1, pp. 44-5, (1989) (PubMed).

Détail du antigène

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Autre désignation: IL1RN (IL1RN Produits)

Synonymes: DIRA Kit ELISA, ICIL-1RA Kit ELISA, IL-1RN Kit ELISA, IL-1ra Kit ELISA, IL-1ra3 Kit ELISA, IL1F3 Kit ELISA, IL1RA Kit ELISA, IRAP Kit ELISA, MVCD4 Kit ELISA, IRAP1 Kit ELISA, IL1RN Kit ELISA, IL-1RA Kit ELISA, F630041P17Rik Kit ELISA, interleukin 1 receptor antagonist Kit ELISA, interleukin 1 receptor antagonist-like Kit ELISA, IL1RN Kit ELISA, Il1rn Kit ELISA, IL1RNL Kit ELISA

Sujet:

Protein Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2, however, the physiological relevance of the latter association is unsure. .

Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Synonyms: Interleukin-1 receptor antagonist protein,IL-1RN,IL-1ra,IRAP,ICIL-1RA,IL1 inhibitor,Anakinra,IL1RN,IL1F3, IL1RA,

Full Gene Name: Interleukin-1 receptor antagonist protein

Cellular Localisation: Isoform 1: Secreted.

ID gène: 3557

UniProt: P18510

Pathways: Signalisation NF-kappaB, Hormone Transport, Cancer Immune Checkpoints