Cholecystokinin Kit ELISA

N° du produit ABIN955818

Détail du produit Cholecystokinin Kit ELISA

Antigène: Cholecystokinin (CCK)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 9.88-800 pg/mL

Seuil minimal de détection: 9.88 pg/mL

Application: ELISA

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human CCK in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human CCK. No significant cross- reactivity or interference between human CCK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human CCK and all the other analogues, therefore, cross reaction may still exist.

Sensibilité: The minimum detectable dose of human CCK is typically less than 3.2 pg/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Attributs du produit: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for human CCK has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled human CCK and unlabeled human CCK (Calibrators or samples) with the pre-coated antibody specific for human CCK. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CCK in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CCK in the sample.

Ingrédients: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Matériel non inclus: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Commentaires:

The calibration curve concentrations used for the ELISA's were 800 pg/mL, 266.67 pg/mL, 88.89 pg/mL, 29.63 pg/mL, 9.88 pg/mL.

Plaque: Pre-coated

Calcul des résultats:

This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between CCK concentration in the sample and the assay signal intensity. Low levels of CCK result in a high O.D. value, while a high concentration of CCK results in a low signal. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log graph paper, with CCK concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Date de péremption: The expiry date is stated on the label.

Détail du antigène

Antigène: Cholecystokinin (CCK)

Abstract: CCK Produits

Synonymes: CCK Kit ELISA, CCK-CH Kit ELISA, cck-a Kit ELISA, cck-b Kit ELISA, cholecystokinin Kit ELISA, cholecystokinin L homeolog Kit ELISA, CCK Kit ELISA, Cck Kit ELISA, cck Kit ELISA, cck.L Kit ELISA

Pathways: TCR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour