IGF2 Kit ELISA
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- Antigène Voir toutes IGF2 Kits ELISA
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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Reactivité
- Boeuf (Vache)
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 3.12-200 ng/mL
- Seuil minimal de détection
- 3.12 ng/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of bovine IGF2 in serum, plasma and other biological fluids.
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of bovine IGF2. No significant cross- reactivity or interference between bovine IGF2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between bovine IGF2 and all the analogues, therefore, cross reaction may still exist.
- Sensibilité
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The minimum detectable dose of bovine IGF2 is typically less than 1.24 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. value of 20 replicates of the zero calibrator plus three standard deviations. - Attributs du produit
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF2. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of IGF2 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- Ingrédients
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - Matériel non inclus
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Commentaires
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The calibration curve concentrations used for the ELISA's were 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL.
- Plaque
- Pre-coated
- Calcul des résultats
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Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with IGF2 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- Date de péremption
- The expiry date is stated on the label.
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- Antigène Voir toutes IGF2 Kits ELISA
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
- Autre désignation
- IGF2 (IGF2 Produits)
- Synonymes
- C11orf43 Kit ELISA, IGF-II Kit ELISA, PP9974 Kit ELISA, LOC100136550 Kit ELISA, igf2 Kit ELISA, igf2b Kit ELISA, wu:fc26e03 Kit ELISA, zgc:91781 Kit ELISA, igf-2 Kit ELISA, insigf Kit ELISA, pp9974 Kit ELISA, AL033362 Kit ELISA, Igf-2 Kit ELISA, Igf-II Kit ELISA, M6pr Kit ELISA, Mpr Kit ELISA, Peg2 Kit ELISA, IGFII Kit ELISA, RNIGF2 Kit ELISA, IGF-II-B Kit ELISA, igf2-a Kit ELISA, igf2-b Kit ELISA, insulin like growth factor 2 Kit ELISA, insulin-like growth factor II Kit ELISA, insulin-like growth factor 2a Kit ELISA, insulin like growth factor 2 S homeolog Kit ELISA, insulin-like growth factor 2 Kit ELISA, insulin-like growth factor 2 (somatomedin A) Kit ELISA, insulin like growth factor 2 L homeolog Kit ELISA, IGF2 Kit ELISA, LOC100136550 Kit ELISA, igf2a Kit ELISA, igf2.S Kit ELISA, Igf2 Kit ELISA, igf2.L Kit ELISA
- Pathways
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
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