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IL-5 Kit ELISA

IL5 Reactivité: Rat Colorimetric Sandwich ELISA 15.6-1000 pg/mL
N° du produit ABIN955911
  • Antigène Voir toutes IL-5 (IL5) Kits ELISA
    IL-5 (IL5) (Interleukin 5 (IL5))
    Reactivité
    • 12
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat IL5 in serum, plasma and other biological fluids.
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of rat IL5. No significant cross- reactivity or interference between rat IL5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between rat IL5 and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    The minimum detectable dose of rat IL5 is typically less than 6.3 pg/mL.
    The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.
    Attributs du produit
    The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL5. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of IL5 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
    Ingrédients
    Pre-coated, ready to use 96-well strip plate (1x)
    Calibrator (lyophilized) (2x)
    Calibrator Diluent (1 x 20 mL)
    Detection Reagent A (1 x 120 µL)
    Detection Reagent B (1 x 120 µL)
    Assay Diluent A (2X concentrate) (1 x 6 mL)
    Assay Diluent B (2X concentrate) (1 x 6 mL)
    TMB Substrate (1 x 9 mL)
    Stop Solution (1 x 6 mL)
    Wash Buffer (30X concentrate) (1 x 20 mL)
    Plate sealer for 96 wells (4x).
    Matériel non inclus
    1. Microplate reader with 450 +/- 10 nm filter.
    2. Precision single or multi-channel pipettes with disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. De-ionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Commentaires

    The calibration curve concentrations used for the ELISA's were 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.2 pg/mL, 15.6 pg/mL.

    Plaque
    Pre-coated
    Calcul des résultats

    Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with IL5 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
    Date de péremption
    The expiry date is stated on the label.
  • Antigène Voir toutes IL-5 (IL5) Kits ELISA
    IL-5 (IL5) (Interleukin 5 (IL5))
    Abstract
    IL5 Produits
    Synonymes
    EDF Kit ELISA, IL-5 Kit ELISA, TRF Kit ELISA, Il-5 Kit ELISA, IL5 Kit ELISA, interleukin 5 Kit ELISA, IL5 Kit ELISA, Il5 Kit ELISA
    Pathways
    Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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