When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
HSPB8
(Heat Shock 22kDa Protein 8 (HSPB8))
Synonymes
CMT2L Peptide, DHMN2 Peptide, E2IG1 Peptide, H11 Peptide, HMN2 Peptide, HMN2A Peptide, HSP22 Peptide, MGC64408 Peptide, hsc70 Peptide, wu:fb01g06 Peptide, wu:fi48b06 Peptide, fc09c11 Peptide, wu:fc04b04 Peptide, wu:fc09c11 Peptide, zgc:64202 Peptide, AU018630 Peptide, AW413033 Peptide, Cryac Peptide, D5Ucla4 Peptide, H11K Peptide, HSP20-like Peptide, Hsp22 Peptide, heat shock protein family B (small) member 8 Peptide, heat shock protein family B (small) member 8 L homeolog Peptide, heat shock protein 8 Peptide, heat shock protein b8 Peptide, HSPB8 Peptide, hspb8.L Peptide, hspa8 Peptide, hspb8 Peptide, Hspb8 Peptide