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alpha-conotoxin IMI peptide (Atto 590)

Reactivité: Conus imperialis Hôte: Synthetic FM, IF, LCI
N° du produit ABIN7582096
710,62 €
Plus frais de livraison 40,00 € et TVA
Destination: France
Envoi sous 8 à 9 jours ouvrables

Aperçu rapide pour alpha-conotoxin IMI peptide (Atto 590) (ABIN7582096)

Antigène

alpha-conotoxin IMI

Origine

Conus imperialis

Source

  • 1
Synthetic

Application

Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
  • Type de proteíne

    Synthetic

    Activité biologique

    Active

    Purification/Conjugué

    Atto 590

    Fonction

    α-Conotoxin ImI-ATTO Fluor-590

    Séquence

    GCCSDPRCAW RC-NH2

    Specificité

    Toxin target: α3β2 and α7 nAChRs

    Attributs du produit

    ATTO 590 (Ex/Em: 593/622 nm). The extent of labeling is 1 Molecule of dye per molecule of i±-Conotoxin ImI, linked via primary amines.

    Biological Activity Comment

    α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
  • Indications d'application

    Antigen Preadsorption Control: 1 - 2 μM

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

    Buffer

    Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

    Conseil sur la manipulation

    Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

    Stock

    -20 °C

    Stockage commentaire

    The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
  • Antigène

    alpha-conotoxin IMI

    Sujet

    A fluorescent probe for imaging and sensitive detection of α3β2 and α7 nicotinic acetylcholine receptors (nAChR)

    Poids moléculaire

    1924 Da
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