GM-CSF Protein (AA 18-144)

N° du produit ABIN1096770

Cette protéine Recombinant GM-CSF est produite dans Yeast (Pichia pastoris).

Aperçu rapide pour GM-CSF Protein (AA 18-144) (ABIN1096770)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Yeast (Pichia pastoris)

Application: Functional Studies (Func)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 18-144

Fonction: Recombinant Human GM-CSF/CSF2 (P. pastoris)

Séquence: APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Attributs du produit: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor/GM-CSF is produced with our Pichia pastoris expression system. The target protein is expressed with sequence (Ala18-Glu144) of Human GM-CSF.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Commentaires:

Biological activity: ED50 is less than 0.2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.Specific Activity of 5.0 x 106 IU/ mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 10 mM TrisHCl, 4 % Mannitol, 1 % Sucrose, pH 8.5.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages, and eosinophils, GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
Alternative Names: Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, Colony-Stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF

Poids moléculaire: 14.4 kDa

UniProt: P04141

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin