Vinculin Protein (VCL) (AA 2-1066)

N° du produit ABIN1097791

Cette protéine Recombinant Vinculin est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Vinculin Protein (VCL) (AA 2-1066) (ABIN1097791)

Antigène: Vinculin (VCL)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 2-1066

Fonction: Recombinant Human Vinculin

Séquence: PVFHTRTIES ILEPVAQQIS HLVIMHEEGE VDGKAIPDLT APVAAVQAAV SNLVRVGKET VQTTEDQILK RDMPPAFIKV ENACTKLVQA AQMLQSDPYS VPARDYLIDG SRGILSGTSD LLLTFDEAEV RKIIRVCKGI LEYLTVAEVV ETMEDLVTYT KNLGPGMTKM AKMIDERQQE LTHQEHRVML VNSMNTVKEL LPVLISAMKI FVTTKNSKNQ GIEEALKNRN FTLEKMSAEI NEIIRVLQLT SWDEDAWASK DTEAMKRALA SIDSKLNQAK GWLRDPSASP GDAGEQAIRQ ILDEAGKVGE LCAGKERREI LGTCKMLGQM TDQVADLRAR GQGSSPVAMQ KAQQVSQGLD VLTAKVENAA RKLEAMTNSK QSIAKKIDAA QNWLADPNGG PEGEEQIRGA LAEARKIAEL CDDPKERDDI LRSLGEISAL TSKLADLRRQ GKGDSPEARA LAKQVATALQ NLQTKTNRAV ANSRPAKAAV HLEGKIEQAQ RWIDNPTVDD RGVGQAAIRG LVAEGHRLAN VMMGPYRQDL LAKCDRVDQL TAQLADLAAR GEGESPQARA LASQLQDSLK DLKARMQEAM TQEVSDVFSD TTTPIKLLAV AATAPPDAPN REEVFDERAA NFENHSGKLG ATAEKAAAVG TANKSTVEGI QASVKTAREL TPQVVSAARI LLRNPGNQAA YEHFETMKNQ WIDNVEKMTG LVDEAIDTKS LLDASEEAIK KDLDKCKVAM ANIQPQMLVA GATSIARRAN RILLVAKREV ENSEDPKFRE AVKAASDELS KTISPMVMDA KAVAGNISDP GLQKSFLDSG YRILGAVAKV REAFQPQEPD FPPPPPDLEQ LRLTDELAPP KPPLPEGEVP PPRPPPPEEK DEEFPEQKAG EVINQPMMMA ARQLHDEARK WSSKGNDIIA AAKRMALLMA EMSRLVRGGS GTKRALIQCA KDIAKASDEV TRLAKEVAKQ CTDKRIRTNL LQVCERIPTI STQLKILSTV KATMLGRTNI SDEESEQATE MLVHNAQNLM QSVKETVREA EAASIKIRTD AGFTLRWVRK TPWYQ

Attributs du produit: Recombinant Human Vinculin is produced by our E. coli expression system. The target protein is expressed with sequence (Pro2-Gln1066) of Human Vinculin.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: Vinculin (VCL)

Autre désignation: Vinculin

Sujet: Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/alpha-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.
Alternative Names: Vinculin, Metavinculin, VCL

Poids moléculaire: 117 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2