TDG Protein (AA 1-410) (His tag)

N° du produit ABIN1098673

Protéine Recombinant TDG exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TDG Protein (AA 1-410) (His tag) (ABIN1098673)

Antigène: TDG (Thymine-DNA Glycosylase (TDG))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 85 %

Détail du produit

Attributs du protein: AA 1-410

Purification/Conjugué: Cette TDG protéine est marqué à la His tag.

Séquence: MEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA

Purification: > 85% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Synonyms: Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4 urea, 10 % glycerol, 0.1M NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: TDG (Thymine-DNA Glycosylase (TDG))

Autre désignation: TDG

Sujet: Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E. coli

Poids moléculaire: 48.4 kDa (433aa)

Pathways: Réparation de l'ADN, Chromatin Binding