TDG Protein (His tag)
Aperçu rapide pour TDG Protein (His tag) (ABIN1098673)
Antigène
Voir toutes TDG ProtéinesType de proteíne
Origine
Source
Application
Pureté
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Purification/Conjugué
- Cette TDG protéine est marqué à la His tag.
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Thymine-DNA Glycosylase (TDG) (AA 1-421) protein (His tag)
custom-made TDG Origine: Souris Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Thymine-DNA Glycosylase (TDG) (AA 1-410) protein (His tag)custom-made TDG Origine: Humain Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Thymine-DNA Glycosylase (TDG) (AA 1-410) protein (GST tag)TDG Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
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Commentaires
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Synonyms: Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/ml (determined by Bradford assay)
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Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 Urea, 10% glycerol, 0.1 M NaCl
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Stock
- 4 °C
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Stockage commentaire
- Avoid repeated freezing and thawing cycles.
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- TDG (Thymine-DNA Glycosylase (TDG))
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Autre désignation
- TDG
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Sujet
- Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E.coli
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Poids moléculaire
- 48.4 kDa (433aa)
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Pathways
- Réparation de l'ADN, Chromatin Binding
Antigène
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