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VEGF 165 (AA 207-371) Protéine

Protéine Recombinant exprimée dans Escherichia coli (E. coli).
N° du produit ABIN1098802

Aperçu rapide pour VEGF 165 (AA 207-371) Protéine (ABIN1098802)

Antigène

VEGF 165

Type de proteíne

Recombinant

Origine

  • 23
  • 2
  • 2
Humain

Source

  • 12
  • 8
  • 2
  • 2
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Pureté

> 90% by SDS-PAGE
  • Attributs du protein

    AA 207-371

    Séquence

    MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

    Purification

    purified by chromatography

    niveau d'endotoxine

    < 1 EU per 1ug of protein (determined by LAL method)
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  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 0.1 MM PMSF, 1 MM DTT

    Stock

    4 °C,-20 °C,-80 °C

    Stockage commentaire

    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
  • Antigène

    VEGF 165

    Sujet

    Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

    Poids moléculaire

    19.3 kDa (166aa) confirmed by MALDI-TOF

    NCBI Accession

    NP_001020539
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