PDGF-BB Homodimer (Homodimer) (Active) Protéine

N° du produit ABIN1589629

Protéine Recombinant exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour PDGF-BB Homodimer (Homodimer) (Active) Protéine (ABIN1589629)

Antigène: PDGF-BB Homodimer

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE

Détail du produit

Attributs du protein: Homodimer

Fonction: PDGF-BB

Séquence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Specificité: Chromosomal location:22q13.1

Attributs du produit: Length (aa):109

niveau d'endotoxine: < 0.1 ng per ug of PDGF-BB

Information d'application

Indications d'application: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: RT,-20 °C,-80 °C

Stockage commentaire: The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-BB is best stored at -20°C to -70°C.

Référence

Rezzola, Dal Monte, Belleri, Bugatti, Chiodelli, Corsini, Cammalleri, Cancarini, Morbidelli, Oreste, Bagnoli, Semeraro, Presta: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." dans: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).

Détail du antigène

Antigène: PDGF-BB Homodimer

Autre désignation: PDGF-BB

Sujet: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, INN=Becaplermin

Poids moléculaire: 24.3 kDa

ID gène: 5155

NCBI Accession: NM_002608, NP_002599

UniProt: P01127