GM-CSF Protéine

N° du produit ABIN1589646

Cette protéine Recombinant GM-CSF est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour GM-CSF Protéine (ABIN1589646)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 98 % by SDS-PAGE

Détail du produit

Fonction: GM-CSF

Séquence: APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Specificité: Chromosomal location:5q31.1

Attributs du produit: Length (aa):127

niveau d'endotoxine: < 0.1 ng per μg of GM-CSF

Information d'application

Indications d'application: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1 ng/mL corresponding to a specific activity of ≥1 x 10^7 units/mg.

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized rh GM-CSF is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: PBS, pH 7.2

Stock: RT,-20 °C

Stockage commentaire: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted GM-CSF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5.
Synonyms: CSF2, GM-CSF

Poids moléculaire: 14.5 kDa

ID gène: 1437

NCBI Accession: NM_000758, NP_000749

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin