Podoplanin Protein (PDPN) (Soluble) (His tag)

N° du produit ABIN1589760

Cette protéine Recombinant Podoplanin est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Podoplanin Protein (PDPN) (Soluble) (His tag) (ABIN1589760)

Antigène: Podoplanin (PDPN)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE

Détail du produit

Attributs du protein: Soluble

Purification/Conjugué: Cette Podoplanin protéine est marqué à la His tag.

Fonction: Podoplanin, soluble

Séquence: GSSHHHHHHS SGLVPRGSHM EGASTGQPED DTETTGLEGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHsTE KVDGDTQTTV EKDGLST

Specificité: Chromosomal location:1p36.21

Attributs du produit: Length (aa):127

Information d'application

Commentaires:

Soluble Receptors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.

Buffer: PBS

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: RT,-20 °C

Stockage commentaire: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted sPodoplanin should be stored in working aliquots at -20°C.

Détail du antigène

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin

Sujet: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosylation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth. The recombinant soluble Podoplanin starts with GLST and ends with GLST.
Synonyms: PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26

Poids moléculaire: 13.1 kDa

ID gène: 10630

NCBI Accession: NM_001006624, NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport