IL-17 Protéine

N° du produit ABIN1684755

Cette protéine IL-17 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-17 Protéine (ABIN1684755)

Antigène: IL-17 (IL17) (Interleukin 17 (IL17))

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 %, as determined by SDS-PAGE and HPLC

Détail du produit

Specificité: Optimized DNA sequence encoding Mouse Interleukin-17A mature chain was expressed in Escherichia Coli.

Attributs du produit: Native Mouse Interleukin17A, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Mouse IL-17A is a disulfide-linked monodimeric protein consisting of 137 amino acid residue subunits, and migrates as an approximately 16kDa protein under reducing conditions in SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Information d'application

Commentaires:

The activity was measured by the ability to bind Human IL17RA in a functional ELISA assay with a linear range of 10-500 ng/mL. Complete precursor sequence shown, expressed chain highlighted

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: PBS solution pH 7.5.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Date de péremption: 12-24 months

Détail du antigène

Antigène: IL-17 (IL17) (Interleukin 17 (IL17))

Autre désignation: IL-17

Sujet: IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells. IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes.Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL-33 produced a wide spectrum of cytokines and chemokines. In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

UniProt: Q62386