ATG5 Protein (AA 1-275)

N° du produit ABIN1692441

Cette protéine Recombinant ATG5 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour ATG5 Protein (AA 1-275) (ABIN1692441)

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-275

Fonction: Recombinant Human Autophagy Related 5 Homolog/ATG5

Séquence: MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD

Attributs du produit:

Recombinant Human Autophagy Related 5 Homolog/ATG5

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,0.2M NaCl,1 mM DTT,40 % glycerol, pH 8.0 .

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: Autophagy Protein 5/ATG5

Sujet: Recombinant Human Autophagy protein 5/ATG5 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp275) of Human ATG5.
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Poids moléculaire: 32.4 kDa

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy