LIF Protein (AA 23-202)

N° du produit ABIN2018195

Détail du produit

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-202

Origine: Humain

Source: Escherichia coli (E. coli)

Attributs du produit: ED50 < 0.2 ng/mL, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5 x 10^6 units/mg.

Pureté: > 95 % by SDS-PAGE analysis.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C. ,

Date de péremption: 6 months

Référence

Huang, Li, Alonso-Gonzalez, Gorre, Keatley, Green, Turner, Kallingappa, Verma, Oback: "A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition." dans: PLoS ONE, Vol. 6, Issue 9, pp. e24501, (2011) (PubMed).

Yu, Kastin, Pan: "TNF reduces LIF endocytosis despite increasing NFkappaB-mediated gp130 expression." dans: Journal of cellular physiology, Vol. 213, Issue 1, pp. 161-6, (2007) (PubMed).

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Abstract: LIF Produits

Sujet: Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low, and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: D factor, MLPLI

Poids moléculaire: 19.7 kDa, observed by reducing SDS-PAGE.

UniProt: P15018

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding