PDGF-BB Homodimer (AA 74-182) (Active) Protéine

N° du produit ABIN2018325

Protéine Recombinant exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PDGF-BB Homodimer (AA 74-182) (Active) Protéine (ABIN2018325)

Antigène: PDGF-BB Homodimer

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE analysis.

Détail du produit

Attributs du protein: AA 74-182

Attributs du produit: ED50 < 2 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5 x 10^5 units/mg.
AA 74-182, expressed with an N-terminal Met.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in 20 mM acetic acid at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 20 mM acetic acid.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: PDGF-BB Homodimer

Autre désignation: Platelet-Derived Growth Factor-BB (PDGF-BB)

Sujet: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

Poids moléculaire: 24.6 kDa, observed by non-reducing SDS-PAGE.

UniProt: Q05028