FASL Protein (Soluble)

N° du produit ABIN2469071

Cette protéine Recombinant FASL est produite dans CHO Cells.

Aperçu rapide pour FASL Protein (Soluble) (ABIN2469071)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Pureté: <= 95 % by SDS-PAGE gel and HPLC analyses.

Détail du produit

Attributs du protein: Soluble

Séquence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

Attributs du produit: Biological activity was determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is < 10.0 ng/mL, corresponding to a specific activity of > 1 x 10⁵ units/mg.

niveau d'endotoxine: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Conseil sur la manipulation: As with any protein, exposing sFas Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -20 °C

Stockage commentaire: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted sFas Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Date de péremption: 24 months

Détail du antigène

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: Fas Ligand

Sujet: Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.

ID gène: 356

NCBI Accession: NP_000630

OMIM: 4557329

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity