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Amphiregulin Protein (AREG) (AA 94-191)

AREG Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. FACS Active
N° du produit ABIN2666768
  • Antigène Voir toutes Amphiregulin (AREG) Protéines
    Amphiregulin (AREG)
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 94-191
    Origine
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Souris
    Source
    • 17
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    Flow Cytometry (FACS)
    Pureté
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Stérilité
    0.22 μm filtered
    Top Product
    Discover our top product AREG Protéine
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Biological activity: ED50 = 0.04 - 0.2 μg/ml, corresponding to a specific activity of 0.5 - 2.5 x 104 units/mg, as determined by induction of BALB/3T3 cell proliferation.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS, pH 7.2.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Antigène
    Amphiregulin (AREG)
    Autre désignation
    Amphiregulin (AREG Produits)
    Synonymes
    AR Protein, CRDGF Protein, SDGF Protein, Sdgf Protein, AREG Protein, AREGB Protein, AREG/SDGF Protein, amphiregulin Protein, AREG Protein, Areg Protein
    Sujet
    Amphiregulin was initially identified in the conditioned medium of human mammary gland MCF-7 cells treated with TPA. The mouse gene was cloned from the androgen-dependent SC2G cell line derived from Shionogi mouse mammary carcinoma SC115. It belongs to the EGF family of proteins that includes EGF, TGF-α, heparin-binding EGF like-growth factor (HB-EGF), epigen, epiregulin, and betacellulin. Mouse amphiregulin is derived from a 248 amino acid transmembrane precursor, and it has 66 % identity to the human protein. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. ADAM17 (TACE) has an important role in ectodomain shedding of amphiregulin, TNF-α, and HB-EGF. This cleavage is a key step in the control of ligand availability and receptor activation, and it is stimulated by physiological and pharmacological agonists, including TPA, calcium ionophores, GPCR ligands, cytokines, and growth factors. Amphiregulin is a paracrine regulator of estrogen action during ductal morphogenesis (mammary gland development), and it has been associated with breast cancer initiation and progression. Amphiregulin is elevated in psoriatic lesional skin, gastrointestinal carcinomas, colorectal cancer, and hepatocelular carcinoma tissues. In addition, FOXP3+ Tregs express EGFR under inflammatory conditions, and amphiregulin enhances regulatory T cell-suppressive function in vitro and in vivo in a colitis and tumor vaccination model.
    Poids moléculaire
    The 98 amino acid recombinant protein has a predicted molecular mass of approximately 11.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 16 kDa and by SDS-PAGE. The N-terminal amino acid is Ser.
    Pathways
    Signalisation RTK, EGFR Signaling Pathway
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