CXCL12 Protein (AA 22-93)

N° du produit ABIN2666911

Cette protéine Recombinant CXCL12 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour CXCL12 Protein (AA 22-93) (ABIN2666911)

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: ELISA, Flow Cytometry (FACS)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 22-93

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Bioactivity was measured by its ability to chemoattract THP-1 cells in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: CXCL12

Sujet: CXCL12 is commonly known as SDF-1, with two variants, SDF-1α (89 amino acids) and SDF-1β (93 amino acids) derived from alternative splicing. They have identical amino acid sequences, differing only by the addition of four amino acids at the carboxy-terminus of the β form. CXCL12β is constitutively expressed by stromal cells. CXCL12α and β are ubiquitously expressed, and it has been suggested that their activity is regulated through differential proteolytic processing. Proteases present in serum process CXCL12α initially at the carboxy-terminus (through carboxypeptidase N) and subsubsequently at the amino-terminus (through CD26), while CXCL12β is only processed at the amino-terminus. These proteolytic processes affect the chemokine's ability to bind to heparin and cells as well as its ability to stimulate proliferation and chemotaxis. CXCL12α is inactivated by other enzymes such as metalloproteases, serine proteases, and leukocyte elastase. CXCL12/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Also, CXCL12 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. Data suggests that CXCL12β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.

Poids moléculaire: The 72 amino acid recombinant protein has a predicted molecular mass of approximately 8.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 and 14 kDa by SDS-PAGE respectively. The N-terminal amino acid is Lysine.

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling