FLT3LG Protein (AA 27-181)

N° du produit ABIN2667412

Cette protéine Recombinant FLT3LG est produite dans Escherichia coli (E. coli).

Aperçu rapide pour FLT3LG Protein (AA 27-181) (ABIN2667412)

Antigène: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 27-181

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 ≤1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg, as measured by its ability to stimulate the proliferation of human AML5 cells in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Autre désignation: Flt-3 Ligand

Sujet: Fms-like tyrosine kinase 3 ligand is a hematopoietic cytokine structurally similar to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). Flt-3 ligand synergizes with other growth factors, such as interleukin-7 (IL-7) and CSFs, to promote proliferation and differentiation of early hematopoietic cells. In addition, Flt-3 ligand is involved in the commitment and expansion of dendritic cells as well as stimulating B cells, T cells, and NK cells. Both soluble form and membrane-bound forms of Flt-3 ligand are able to stimulate the cells expressing receptor tyrosine kinases Flt-3, transducing signals through the ERK and PI3K pathway. Flt-3 ligand is the only known ligand for Flt-3. Flt-3 and Flt-3 ligand have been implicated to participate in leukemogenesis, since the constitutively activated mutants of Flt-3 were found to be associated with the acute myeloid leukemia (AML).

Poids moléculaire: The 155 amino acid recombinant protein has a predicted molecular mass of approximately 17.6 kDa. The predicted N-terminal amino acid is Thr.

Pathways: Signalisation RTK