GM-CSF Protein (AA 18-141)

N° du produit ABIN2667426

Protéine Recombinant GM-CSF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour GM-CSF Protein (AA 18-141) (ABIN2667426)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 18-141

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 is <0.05 ng/ml, corresponding to a specific activity >2x107 units/mg.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Poids moléculaire: The 125 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kDaa. The DTT-reduced protein migrates at approximately 13 kDaa and the non-reduced protein migrates at approximately 11kDa by SDS-PAGE.

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin