IL1A Protein (AA 113-271)

N° du produit ABIN2667482

Cette protéine Recombinant IL1A est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL1A Protein (AA 113-271) (ABIN2667482)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Intracellular Flow Cytometry (ICFC)

Pureté: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 113-271

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 is 5 - 15 pg/ml, corresponding to a specific activity of 0.6-2.0 x 107 units/mg, as determined by the dose dependent stimulation of D10 cells proliferation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL-1alpha

Sujet: IL-1 was isolated from human blood that had been exposed to a pathogenic bacterium. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damaged joints and influenced liver proteins (2). IL-1α binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1 and -β and IL-1RA can compete for binding to these receptors. However, only IL-1RI, not IL-1RII, is functional because IL-1RII lacks a cytoplasmic domain and is thus unable to transmit signals to downstream steps (3). IL-1α is expressed by cancer cells and promotes angiogenesis and metastasis of pancreatic cancer and human gastric cancer cell lines (4). In addition, IL-1 induced the expression of VEGF in colon cancer (5).

Poids moléculaire: The 159 amino acid recombinant protein has a predicted molecular mass of 18,047 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints