IL-2 Protein (AA 21-169)

N° du produit ABIN2667500

Cette protéine Recombinant IL-2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-2 Protein (AA 21-169) (ABIN2667500)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Pureté: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 21-169

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 is 0.10-0.40 ng/ml, corresponding to a specific activity of 2.5 - 10 x 106 units/mg, as determined by a dose dependent stimulation of HT-2 cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 5 mM NaH2PO4, 5 mM citric acid, 350 mM NaCl, pH 4.0, 1 mM DTT.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL-2

Sujet: IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes.

Poids moléculaire: The 150 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17,231 Da. The DTT-reduced protein and non-reduced protein migrates at approximately 18kDa by SDS-PAGE.

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation