IL-9 Protein (AA 19-144)

N° du produit ABIN2667544

Cette protéine Recombinant IL-9 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-9 Protein (AA 19-144) (ABIN2667544)

Antigène: IL-9 (IL9) (Interleukin 9 (IL9))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Intracellular Flow Cytometry (ICFC), Immunofluorescence (IF)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Détail du produit

Attributs du protein: AA 19-144

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of human M07e cells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: IL-9 (IL9) (Interleukin 9 (IL9))

Autre désignation: IL-9

Sujet: IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.

Poids moléculaire: The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kDa. The predicted N-terminal amino acid is Met.

Pathways: Signalistation JAK/STAT