Thrombopoietin Protein (THPO) (AA 22-195)

N° du produit ABIN2667585

Protéine Recombinant Thrombopoietin exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Thrombopoietin Protein (THPO) (AA 22-195) (ABIN2667585)

Antigène: Thrombopoietin (THPO)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Détail du produit

Attributs du protein: AA 22-195

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The expected ED50 is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg, as determined by the dose-dependent stimulation of the proliferation of human MO7e cells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: Thrombopoietin (THPO)

Autre désignation: Thrombopoietin

Sujet: Mouse Thrombopoietin (TPO) is a protein consisting of an N-terminus domain with homology to erythropoietin (EPO) and a C-terminus domain with multiple N-linked and O-linked glycosylation sites. TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes, hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Poids moléculaire: The 174 amino acid recombinant protein has a predicted molecular mass of 18.7 kDa. The predicted N-terminal amino acid is Ser.

Pathways: Signalistation JAK/STAT, Hormone Activity