Annexin V Protein (Biotin)

N° du produit ABIN2669255

Cette protéine Annexin V est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Annexin V Protein (Biotin) (ABIN2669255)

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Origine: Mammifères

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Détail du produit

Purification/Conjugué: Cette Annexin V protéine est marqué à la Biotin.

Attributs du produit: Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (Cat. No. 422201) is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (Cat. No. 420403/420404) or Propidium Iodide Solution (Cat. No. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Purification: The purified protein was conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin and unconjugated antibody.

Ingrédients: Biotin Annexin V

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from prolonged exposure to light. Do not freeze.

Stock: 4 °C

Stockage commentaire: The Annexin V solution should be stored undiluted between 2°C and 8°C.

Détail du antigène

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: Annexin V

Pathways: Apoptose