CD14 Protein (CD14) (Transcript Variant 1)
-
- Antigène Voir toutes CD14 Protéines
- CD14
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- Transcript Variant 1
-
Origine
- Humain
-
Source
- HEK-293 Cells
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
- Attributs du produit
-
- Recombinant human CD14 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Pureté
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- niveau d'endotoxine
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose dependent activation of NFkB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/uL to 200 ng/uL. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/uL to 1.0 ng/uL bacterial LPS.
- Top Product
- Discover our top product CD14 Protéine
-
-
- Indications d'application
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
-
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Conseil sur la manipulation
- Resuspend the protein in the desired concentration in proper buffer
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Antigène
- CD14
- Autre désignation
- CD14 (CD14 Produits)
- Synonymes
- CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
- Sujet
- The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
- Poids moléculaire
- 36.4 kDa
- NCBI Accession
- NP_000582
- Pathways
- Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
-