IDE Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2723516

Protéine Recombinant IDE exprimée dans HEK-293 Cells et a été mentionnée dans 1 publication.

Aperçu rapide pour IDE Protein (Myc-DYKDDDDK Tag) (ABIN2723516)

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Purification/Conjugué: Cette IDE protéine est marqué à la Myc-DYKDDDDK Tag.

Attributs du produit:

• Recombinant human Insulin-degrading enzyme protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Kim, Lone, Saghatelian: "Analysis of the proteolysis of bioactive peptides using a peptidomics approach." dans: Nature protocols, Vol. 8, Issue 9, pp. 1730-42, (2013) (PubMed).

Détail du antigène

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Autre désignation: Insulin-Degrading Enzyme

Sujet: This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009].

Poids moléculaire: 117.8 kDa

NCBI Accession: NP_004960

Pathways: SARS-CoV-2 Protein Interactome