PAI1 Protein (Transcript Variant 1)

N° du produit ABIN2731826

Détail du produit

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human SERPINE1 / PAI1 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37C. Half maximal inhibition against 1.0 ug/mL of single chain tPA was obtained at a concentration of 2.0ug/mL.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Autre désignation: Serpine1,pai1 (SERPINE1 Produits)

Synonymes: PAI Protein, PAI-1 Protein, PAI1 Protein, PLANH1 Protein, SERPINE1 Protein, si:ch211-138a11.1 Protein, Planh1 Protein, PAI1A Protein, Pai1 Protein, Pai1aa Protein, Planh Protein, RATPAI1A Protein, serpin family E member 1 Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade E, member 1 Protein, SERPINE1 Protein, serpine1.L Protein, serpine1 Protein, Serpine1 Protein

Sujet: This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 42.7 kDa

NCBI Accession: NP_000593

Pathways: Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration