IgE Protéine
Aperçu rapide pour IgE Protéine (ABIN363697)
Antigène
Voir toutes IgE ProtéinesType de proteíne
Origine
Source
Application
Pureté
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Specificité
- Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
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Attributs du produit
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IGHE, chromosome 14q32.33
Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system) -
Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
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IgE (AA 209-428) protein (His-Avi Tag)
Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 208-429) protein (His-Avi Tag)Origine: Cynomolgus Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 91-421) protein (His tag)Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 198-421) protein (His-Avi Tag)Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE protein (Biotin)Origine: Humain Hôte: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-429) protein (His tag)Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)Origine: Cynomolgus Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Indications d'application
- For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS, pH=7.2, 0.1 % NaN3
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Stock
- 4 °C
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- IgE
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Sujet
- Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.
Antigène
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