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Albumin Protein (ALB) (TRITC)

Cette protéine Albumin est exprimée dans Cow.
N° du produit ABIN457572

Aperçu rapide pour Albumin Protein (ALB) (TRITC) (ABIN457572)

Antigène

Voir toutes Albumin (ALB) Protéines
Albumin (ALB)

Origine

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Boeuf (Vache)

Source

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Cow

Application

Staining Methods (StM)

Pureté

> 90 %
  • Purification/Conjugué

    Cette Albumin protéine est marqué à la TRITC.

    Attributs du produit

    Tetramethylrhodamine isothiocyanate-conjugated bovine serum albumin, Cohn fraction V. Source: Pooled normal bovine serum

    Purification

    Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains traces transferrin and some alpha-proteins.
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  • Indications d'application

    As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the orange-red fluorescence of BSA/TRITC. The purpose of counterstaining is to enhance the optical contrast of the immunospecific staining against a counterstained background.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    BSA concentration 11.6 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.0.

    Buffer

    Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2).

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
  • Antigène

    Albumin (ALB)

    Autre désignation

    Serum Albumin

    Sujet

    Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to bovine total serum proteins, to bovine IgA, IgM and albumin

    Pathways

    Lipid Metabolism
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