CD14 Protein (CD14) (His tag)

N° du produit ABIN5663349

Protéine Recombinant CD14 exprimée dans CHO Cells.

Aperçu rapide pour CD14 Protein (CD14) (His tag) (ABIN5663349)

Antigène: CD14

Type de proteíne: Recombinant

Origine: Humain

Source: CHO Cells

Application: Western Blotting (WB), ELISA, Agonist (Agon), Dot Blot (DB)

Pureté: >95 % by SDS-PAGE

Détail du produit

Purification/Conjugué: Cette CD14 protéine est marqué à la His tag.

Séquence: MERASCLLLL LLPLVHVSAT TPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PACARSTLSV G VSGTLVLLQG ARGFA

Attributs du produit: The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

Purification: affinity chromatography with monoclonal CD14 antibody biG2

niveau d'endotoxine: <1EU/μg of recombinant human CD14 as determined by LAL method

Biological Activity Comment: Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5μg/ml) to 6 x 105 CD14+CHO transfectants (FACS)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Concentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.2

Agent conservateur: Without preservative

Stock: RT,-20 °C,-80 °C

Stockage commentaire:

Storage: -20°C or -70°C

Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months. The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long-term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided !!!

Date de péremption: 12 months

Détail du antigène

Antigène: CD14

Autre désignation: CD14

Sujet: Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.The rhuCD14 was produced in human CD14 transfected CHO-cells in serum free medium and has been purified by affinity chromatography with monoclonal antibody to hCD14 biG2at column and controlled by SDS page (90-95 % purity). Before transfection, the complete human CD14-cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.

Poids moléculaire: ~50kDa

ID gène: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades