CNTF Protein (AA 1-200)

N° du produit ABIN6387771

Cette protéine Recombinant CNTF est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CNTF Protein (AA 1-200) (ABIN6387771)

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 % by SDS - PAGE

Détail du produit

Attributs du protein: AA 1-200

Séquence: MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less than or equal to 500 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Autre désignation: CNTF

Sujet: CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 22.9 kDa (200aa), confirmed by MALDI-TOF

NCBI Accession: NP_000605

UniProt: P26441

Pathways: Signalistation JAK/STAT