PTH Protein (AA 32-115)
Aperçu rapide pour PTH Protein (AA 32-115) (ABIN6387772)
Antigène
Voir toutes PTH ProtéinesType de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Attributs du protein
- AA 32-115
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Séquence
- MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
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niveau d'endotoxine
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50ng/ml.
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Parathyroid Hormone (PTH) (Active) protein
PTH Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Parathyroid Hormone (PTH) (AA 32-115) protein (Fc Tag)PTH Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)PTH Origine: Rat Hôte: Escherichia coli (E. coli) > 90 % AcA, CC Active
Parathyroid Hormone (PTH) (AA 32-115) protein (His-IF2DI Tag)PTH Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Parathyroid Hormone (PTH) (AA 1-115) protein (GST tag)PTH Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Parathyroid Hormone (PTH) (AA 32-115) protein (GST tag)PTH Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In phosphate-buffered Saline( pH 7.4) containing 2 mM EDTA
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- PTH (Parathyroid Hormone (PTH))
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Autre désignation
- PTH
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Classe de substances
- Hormone
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Sujet
- Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
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Poids moléculaire
- 9.55 kDa (85aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000306
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UniProt
- P01270
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Pathways
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
Antigène
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