LDHB Protein (AA 1-334)

N° du produit ABIN6387839

Protéine Recombinant LDHB exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour LDHB Protein (AA 1-334) (ABIN6387839)

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 90% by SDS-PAGE

Détail du produit

Attributs du protein: AA 1-334

Séquence: MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl (  pH 8.0) containing 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Autre désignation: Lactate Dehydrogenase B/LDHB

Sujet: LDHB, also known as L-lactate dehydrogenase B chain isoform LDHB, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate. The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 36.6 kDa (334aa) confirmed by MALDI-TOF

NCBI Accession: NP_002291

UniProt: P07195

Pathways: L'effet Warburg