Prolactin Protein (PRL) (AA 30-228)
Aperçu rapide pour Prolactin Protein (PRL) (AA 30-228) (ABIN5777732)
Antigène
Voir toutes Prolactin (PRL) ProtéinesType de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Attributs du protein
- AA 30-228
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Séquence
- MQPLPICSAG DCQTSLRELF DRVVILSHYI HTLYTDMFIE FDKQYVQDRE FMVKVINDCP TSSLATPEDK EQALKVPPEV LLNLILSLVQ SSSDPLFQLI TGVGGIQEAP EYILSRAKEI EEQNKQLLEG VEKIISQAYP EAKGNGIYFV WSQLPSLQGV DEESKILSLR NTIRCLRRDS HKVDNFLKVL RCQIAHQNNC
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niveau d'endotoxine
- < 1.0 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal 3 ng/ml.
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Prolactin (PRL) (Active) protein
PRL Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Prolactin (PRL) protein (His tag)PRL Origine: Mouton Hôte: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Prolactin (PRL) protein (His tag)PRL Origine: Souris Hôte: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Prolactin (PRL) (AA 30-226) protein (His-IF2DI Tag)PRL Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Prolactin (PRL) (AA 30-226) protein (His-IF2DI Tag)PRL Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Prolactin (PRL) (AA 29-227) protein (GST tag)PRL Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Prolactin (PRL) (AA 29-227) protein (Fc Tag)PRL Origine: Humain Hôte: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Prolactin (PRL) (AA 29-227) protein (Fc Tag,AVI tag,Biotin)PRL Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In Phosphate buffered saline ( pH 7.4)
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Prolactin (PRL)
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Autre désignation
- Prolactin
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Sujet
- Prolactin, also known as prl, is a hormone synthesized and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation). Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immuno-regulation. Recombinant mouse Prolactin, was expressed in E.coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 22.7kDa (200aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_035294
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UniProt
- Q9CPQ2
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Pathways
- Signalistation JAK/STAT, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
Antigène
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