PRMT1 Protein (AA 1-353) (His-MBP)

N° du produit ABIN666862

Protéine Recombinant PRMT1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PRMT1 Protein (AA 1-353) (His-MBP) (ABIN666862)

Antigène: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 1-353

Purification/Conjugué: Cette PRMT1 protéine est marqué à la His-MBP.

Séquence: MAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Attributs du produit: Human PRMT1 isoform3, 1-353 aa, Human, His-MBP tagged, Recombinant, E.coli

Purification: > 90% by SDS-PAGE

Biological Activity Comment: Specific activity is > 10 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group on histone H4 per minute at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 40 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl, 4 mM MgCl₂, 2 mM DTT, 40 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Autre désignation: PRMT1

Sujet: Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 84.2 kDa (750aa)

NCBI Accession: NP_938074