BAG1 Protein (GST tag)

N° du produit ABIN6699543

N° du produit (Fournisseur): 009-001-r46s

Protéine Recombinant BAG1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour BAG1 Protein (GST tag) (ABIN6699543)

Antigène: BAG1 (BCL2-Associated Athanogene (BAG1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >85%

Détail du produit

Purification/Conjugué: Cette BAG1 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-r46s

Fournisseur: Rockland

Fonction: BAG1 recombinant protein-GST fusion protein

Purification: Recombinant human BAG1 (72-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Information d'application

Indications d'application:

Application Note: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. BAG1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: BAG1 (BCL2-Associated Athanogene (BAG1))

Autre désignation: BAG1

Sujet:

Synonyms: BAG family molecular chaperone regulator 1 (BAG-1) (Bcl-2-associated athanogene 1), HAP, RAP46

Background: BAG1 (also known as BCL2-associated athanogene) is a membrane protein rich in glutamic acid residues that binds to BCL2 and blocks apoptosis or programmed cell death (1). The BAG1-BCL2 complex enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms (2). Overexpression of BAG1 in 3T3 fibroblasts prevents apoptosis in the presence of low serum. BAG1 has also been shown to interact with activated glucocorticoid, androgen, estrogen and progesterone receptors. Binding to these receptors by BAG1 is dependent on receptor activation. BAG1 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_004323