CCL19 Protéine

N° du produit ABIN6699624

N° du produit (Fournisseur): 009-001-c21

Protéine Recombinant CCL19 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CCL19 Protéine (ABIN6699624)

Antigène: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 009-001-c21

Fournisseur: Rockland

Fonction: MIP-3ß Human Recombinant Protein

Purification: Recombinant protein corresponds to amino acids 22 to 98 of mature human MIP-3ß. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by RP- HPLC, against known standard.

niveau d'endotoxine: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Macrophage Inflammatory Protein 3ß is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract human T cells using a concentration of 7 - 40 ng/ml.

Information d'application

Indications d'application:

Application Note: MIP-3ß Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-3ß in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Autre désignation: CCL19

Sujet:

Synonyms: C-C motif chemokine 19, Small-inducible cytokine A19, Macrophage inflammatory protein 3 beta, MIP-3-beta, MIP3B, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1 ligand chemokine, ELC, Beta-chemokine exodus-3, CK beta-11, SCYA19

Background: The MIP-3ß (also known as SCYA19) member of the intercrine beta (chemokine CC) family may play a role not only in inflammatory and immunological responses, but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. It is secreted. It specifically binds to chemokine receptor CCR7. MIP3ß is expressed at high levels in the lymph nodes, thymus and appendix, and at intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. Recombinant Human MIP-3b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Daltons. The recombinant protein shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.

UniProt: Q99731

Pathways: Positive Regulation of Immune Effector Process