CXCL12 Protéine

N° du produit ABIN6699684

N° du produit (Fournisseur): 009-001-w22-0100

Protéine Recombinant CXCL12 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CXCL12 Protéine (ABIN6699684)

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 009-001-w22-0100

Fournisseur: Rockland

Fonction: Human Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein

Purification: Stromal Cell-Derived Factor-1 alpha (CXCL12) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract human peripheral T cells at 10 - 75 ng/mL.

Information d'application

Indications d'application:

Application Note: Stromal Cell-Derived Factor-1 alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stromal Cell-Derived Factor-1 alpha in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: CXCL12

Sujet:

Synonyms: C-X-C motif chemokine 12, Intercrine reduced in hepatomas (IRH, hIRH), Pre-B cell growth-stimulating factor (PBSF)

Background: Stromal cell Derived Factor-1 alpha (SDF-1α), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1α signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human and mouse SDF-1α share 99 % sequence identity. Recombinant human SDF-1α is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 8 kDa.

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling