Cyclin A Protein (GST tag)

N° du produit ABIN6699735

N° du produit (Fournisseur): 009-001-r76s

Protéine Recombinant Cyclin A exprimée dans Insect cells (Sf9).

Aperçu rapide pour Cyclin A Protein (GST tag) (ABIN6699735)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Type de proteíne: Recombinant

Origine: Humain

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Pureté: >75%

Détail du produit

Purification/Conjugué: Cette Cyclin A protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-r76s

Fournisseur: Rockland

Fonction: CyclinA2 recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinA2 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Information d'application

Indications d'application:

Application Note: CyclinA2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinA2 Protein is suitable for use in Western Blot. Expect a band approximately ~ 78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: CyclinA2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: CCNA2

Sujet:

Synonyms: CCNA2, CCN1, CCNA, Cyclin-A2, Cyclin-A

Background: CyclinA2 belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle and function as regulators of CDKs. CyclinA2 is an essential component of all embryonic and somatic cell cycles in mammals (1). CyclinA2 is highly expressed in the testis and certain myeloid leukemia cells (2). Cyclin A also plays a major role in the control of DNA replication (3). CyclinA2 binds and activates CDC2 or CDK2 and thus promotes both cell cycle G1/S and G2/M transitions. CyclinA2 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_001237

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA