FGF21 Protéine

N° du produit ABIN6699850

N° du produit (Fournisseur): 009-001-u77-0005

Cette protéine Recombinant FGF21 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour FGF21 Protéine (ABIN6699850)

Antigène: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 95,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u77-0005

Fournisseur: Rockland

Fonction: Human Fibroblast Growth Factor-21 Recombinant Protein

Purification: Fibroblast Growth Factor-21 purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of NIH 3T3 cells in the presence of 5 μg/mL FGF binding protein and is typically 0.12-0.6 μg/mL.

Information d'application

Indications d'application:

Application Note: Fibroblast Growth Factor-21 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-21 in immunological assays. Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Lyophilized in 0.1M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Autre désignation: FGF21

Sujet:

Synonyms: FGFL

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-21 is most closely related to FGF-19 and shows low binding to heparin and is highly diffusible. Recombinant human FGF-21 is a non-glycosylated protein, containing 182 amino acids, with a molecular weight of 19.5 kDa.

UniProt: Q9NSA1

Pathways: Signalisation RTK