IL-2 Protéine

N° du produit ABIN6700063

N° du produit (Fournisseur): 009-001-b95

Cette protéine Recombinant IL-2 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-2 Protéine (ABIN6700063)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 90,00%

Détail du produit

N° du produit (Fournisseur): 009-001-b95

Fournisseur: Rockland

Fonction: IL-2 Human Recombinant Protein

Purification: Purity was determined to be greater than 90% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE, against known standard.

niveau d'endotoxine: Measured by LAL is < 0.5 EU/μg protein.

Biological Activity Comment: Recombinant Human IL-2 is fully bioactive when compared to standard. The ED50 as measured by the dose-dependent proliferation of CTLL-2 cells is typically 1.0 ng/ml.

Information d'application

Indications d'application:

Application Note: IL-2 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-2 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2

Sujet:

Synonyms: Interleukin-2 cytokine, aldesleukin, T cell growth factor2, TCGF,

Background: Interleukin-2 (IL2) is a secreted immunomodulatory cytokine that is essential in regulation and proliferation of T and B lymphocytes, and other activities crucial to regulation of the immune response. The receptor of this cytokine (the IL-2R receptor) is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Recombinant IL-2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15,517 Daltons. Recombinant human IL-2 has a Ser substitute for Cysteine at position 126.

UniProt: Q6QWN0

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation