IL-5 Protéine

N° du produit ABIN6700148

N° du produit (Fournisseur): 010-001-v42-0005

Cette protéine Recombinant IL-5 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-5 Protéine (ABIN6700148)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 97,00%

Détail du produit

N° du produit (Fournisseur): 010-001-v42-0005

Fournisseur: Rockland

Fonction: Mouse Interleukin-5 Recombinant Protein

Purification: Interleukin-5 purity was determined to be greater than 97% as determined by HpLC and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent induction of TF-1 cell proliferation and is typically less than 2 ng/mL.

Information d'application

Indications d'application:

Application Note: Interleukin-5 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-5 in immunological assays. Lyophilized in 20 mM sodium bicarbonate, pH 9.0.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Lyophilized in 20 mM sodium bicarbonate, pH 9.0.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: Il5

Sujet:

Synonyms: EDF, B-cell growthfactor II (BCDFII), Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor (TRF)

Background: Interleukin-5 (IL-5) is a hematopoietic growth factor expressed in Th2, mast cells and eosinophils. IL-5 acts through its receptor, the IL-5 receptor (IL-5R) and is involved in B cell growth and eosinophil activation. IL-5 has been shown to be and is regulated by GATA-3, in addition to other transcription factors. Human and mouse IL-5 are cross-reactive. Recombinant mouse IL-5 is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 26.2 kDa.

UniProt: P04401

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour