Retinoblastoma 1 Protein (RB1) (GST tag)

N° du produit ABIN6700479

N° du produit (Fournisseur): 009-001-t17s

Protéine Recombinant Retinoblastoma 1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Retinoblastoma 1 Protein (RB1) (GST tag) (ABIN6700479)

Antigène: Retinoblastoma 1 (RB1)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >90%

Détail du produit

Purification/Conjugué: Cette Retinoblastoma 1 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-t17s

Fournisseur: Rockland

Fonction: Rb recombinant protein-GST fusion protein

Purification: Recombinant human Rb (48 - 378) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Information d'application

Indications d'application:

Application Note: Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Rb Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~62 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: RB1

Sujet:

Synonyms: RB1, OSRC, pRb, pp110, p105-Rb, Retinoblastoma 1

Background: Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets. Rb Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Cancer, Cell Cycle, and Neurobiology research.

NCBI Accession: NM_000321

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy