SIRT5 Protein (GST tag)

N° du produit ABIN6700535

N° du produit (Fournisseur): 009-001-t35s

Cette protéine Recombinant SIRT5 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour SIRT5 Protein (GST tag) (ABIN6700535)

Antigène: SIRT5 (Sirtuin 5 (SIRT5))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >95%

Détail du produit

Purification/Conjugué: Cette SIRT5 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-t35s

Fournisseur: Rockland

Fonction: SIRT5 recombinant protein-GST fusion protein

Purification: Full-length recombinant human SIRT5 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Information d'application

Indications d'application:

Application Note: SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT5 Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SIRT5 (Sirtuin 5 (SIRT5))

Autre désignation: SIRT5

Sujet:

Synonyms: SIR2L5, Sirtuin 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIRT5, SIR2L5

Background: SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation (1). SIRT5 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_012241

Pathways: SARS-CoV-2 Protein Interactome