BAFF Protéine

N° du produit ABIN6700762

N° du produit (Fournisseur): 009-001-u65-0020

Cette protéine Recombinant BAFF est produite dans Escherichia coli (E. coli).

Aperçu rapide pour BAFF Protéine (ABIN6700762)

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 90,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u65-0020

Fournisseur: Rockland

Fonction: Human BAFF

Purification: BAFF purity was determined to be greater than 90% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a RPMI-8226 cell survival assay in the presence of 0.2 μM DEX and is typically less than 30 ng/mL.

Information d'application

Indications d'application:

Application Note: Human BAFF Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-BAFF in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Autre désignation: TNFSF13B

Sujet:

Synonyms: THANK, B lymphocyte stimulator (BLyS), B-cell activating factor, TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1), CD_antigen=CD257

Background: Human B cell Activating Factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. BAFF initiates signaling through three receptors with human BAFF having activity on mouse splenocytes. Recombinant human BAFF is a non-glycosylated protein containing a N terminal His tag, a thrombin cut site and 153 amino acids of the TNF-like domain. This protein has a total molecular weight of 18.5 kDa.

UniProt: Q9Y275

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response