YWHAE Protein (GST tag)

N° du produit ABIN6700778

N° du produit (Fournisseur): 009-001-r25s

Cette protéine Recombinant YWHAE est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour YWHAE Protein (GST tag) (ABIN6700778)

Antigène: YWHAE (14-3-3 epsilon (YWHAE))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >90%

Détail du produit

Purification/Conjugué: Cette YWHAE protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-r25s

Fournisseur: Rockland

Fonction: 14-3-3 epsilon recombinant protein-GST fusion protein

Purification: Recombinant full-length human 14-3-3ε was expressed in E.coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Information d'application

Indications d'application:

Application Note: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: YWHAE (14-3-3 epsilon (YWHAE))

Autre désignation: YWHAE

Sujet:

Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465

Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.

NCBI Accession: NM_006761

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase